Under the age of we media, the crisis of network public opinion and the characteristics of public opinion subject are closely related From the perspective of inducement analysis of university network public opinion, college students, as the main transmitters of university network public opinion, show characteristics of the game between rational cognition and perceptual representation, deviation of self-identity and social identity, conflicts between individualism and group conformity, self-realization and marginalized situation and coexistence of curiosity and entertainment attitude, all of which can cause the crises of network public opinion under the amplification and fermentation of media And the formation of these characteristics are jointly decided by social system, the multi-cultural background, long-term lack of the public right of discourse, and the identity crisis caused by conversion between real and visual space To give good guidance to the network public opinion under the age of we media, on the basis of analyzing and grasping its subject characteristics and formation, universities must set up the “with thin wall” interactive dialogue mechanism, the “nurture” public opinion guidance mechanism, the “feeling into the principle” multiple education mechanism and “rationality and introspection” self-adjusting mechanism, in order to form multiple levels of education and guide direction of the subject of network public opinion, and finally create open, tolerant and equal network public opinion environment in universities